水質検査における酵素基質培地の適用と本培地により検出された大腸菌群の菌種の検討

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  • Evaluation of Fluorogenic and Chromogenic Substrate Medium for Detection of Coliform Bacteria in Potable Water and Investigation of Genus and Species of the Bacteria Isolated through This Medium
  • スイシツ ケンサ ニ オケル コウソ キシツ バイチ ノ テキヨウ ト ホンバイチ ニ ヨリ ケンシュツ サレタ ダイチョウキングン ノ キンシュ ノ ケントウ

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Test samples of water presented for the potability test were examined in XGM medium, and using the 112 samples that indicated positive for the coliform bacteria group, the results were compared with those obtained with LB medium. Furthermore, the strains of the coliform bacteria group isolated from XGM medium were subjected to identification tests to determine the bacterial type, and the characteristics of the strains isolated were also examined in the LB medium. With respect to the status of detection of organisms of the coliform bacteria group from potable water in each fiscal year, significant differences were noted depending on the test method employed. When the test results for the 112 samples that indicated positive for the coliform bacteria group in XGM medium were compared with those obtained in LB medium, the agreement rate was only 65.2%. When strains of the coliform bacteria group isolated in the XGM medium were subjected to identification tests to determine the bacterial type, the following results were obtained: Enterobacter represented 29.6%, Citrobacter 25.9%, Escherichia 13.2%, Aeromonas 11.6%, Klebsiella 7.9%, and Serratia 5.3%. Out of the 189 isolated strains of the coliform bacteria group isolated from the XGM medium, 114 strains showed positive result in LB medium, and the agreement rate was 60.3%.

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