Evaluation of a Rapid <I>Staphylococcus aureus</I> Detection Kit “KAKUSAN Test <I>Staphyloc occus aureus</I>” by the Colorimetric DNA/rRNA Hybridization Method in Microplate Wells

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  • マイクロプレートＤＮＡ／ｒＲＮＡサンドイッチハイブリダイゼーション法を用いた黄色ブドウ球菌簡易検出キット「核さんテスト　黄色ブドウ球菌」の評価
  • マイクロ プレート DNA rRNA サンドイッチハイブリダイゼーションホウ オ モチイタ キイロ ブドウ キュウキン カンイ ケンシュツ キット カク サン テスト キイロ ブドウ キュウキン ノ ヒョウカ

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A rapid and readily-available DNA probe kit “KAKUSAN Test Staphylococcus aureus” was developed for the detection of Staphylococcus aureus. This kit utilized the colorimetric DNA/rRNA sandwich hybridization method in microplate wells. In this paper, we evaluated and compared this kit with conventional culture methods.<BR>The detection limits of the kits were 7.0×105 cfu/ml in pure culture and 3.2×106cfu/ml in 10% homogenate of commercial foods. The detectability of the kit for 10% homogenate which was sterilized by heating or stored frozen after heating was equal to untreated 10% homogenate.<BR>The ECDP method combined enriched culture with assay by the DNA probe kit. The examination period was shorter than the enriched culture method by two days, but the detectability of these methods were equal.<BR>A total of 273 naturally contaminated samples (commercial lunches, frozen foods and other foods) were tested by direct plate culture method, enriched culture method and the ECDP method. Of these 52 were positive by the ECDP method and enriched culture method. The method agreement rate of the ECDP method and enriched culture method was 100%. All 273 were negative by direct plate culture method.<BR>Those results show that the DNA probe kit and the ECDP method are useful tool to examine various foods for contamination by S. aureus.

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