Confirmation of Effectiveness of Petrifilm EB Plate for <i>Enterobacteriaceae</i> Using Domestic Commercialized Food Products

SAITO Akemi, HARADA Yasuko, KITAHARA Akio, MORIYAMA Takatoshi, TANAKA Hiroyuki

doi:10.5803/jsfm.28.167

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市販食品を用いた<i>Enterobacteriaceae</i> (腸内細菌科)測定用ペトリフィルムEBプレートの有効性の確認
市販食品を用いたEnterobacteriaceae(腸内細菌科)測定用ペトリフィルムEBプレートの有効性の確認
シハンショクヒンオモチイタ Enterobacteriaceae チョウナイサイキンカソクテイヨウペトリフィルム EB プレートノユウコウセイノカクニン
Confirmation of Effectiveness of Petrifilm EB Plate for Enterobacteriaceae Using Domestic Commercialized Food Products

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Abstract

Petrifilm Enterobacteriaceae Count Plate (Petrifilm EB) was evaluated by comparing with ISO method in terms of enumeration of Enterobacteriaceae using domestic commercialized meat products, fish and seafood products, and prepared products. The number of food products was 30 for each category and the total number was 90. The incubation temperature of Petrifilm EB was 37°C and 35°C. There was a high correlation between Petrifilm EB incubated at 37°C and ISO method count results. Two sample t-test result shows it was not statistically significant at the significance level of 5% and Petrifilm EB was comparable to ISO method. There was also a high correlation between Petrifilm EB incubated at 35°C and ISO method count results. However, The Enterobacteriaceae count results of Petrifilm EB incubated at 35°C tended to be higher than of ISO method. Two sample t-test result shows it had significant difference at the significance level of 5% for meat products, and fish and seafood products. There was a high correlation between Petfrifilm EB 35°C and Petfrifilm EB 37°C incubation results. However, the Enterobacteriaceae count results of Petrifilm EB incubated at 35°C tended to be higher than of Petrifilm EB incubated at 37°C. Two sample t-test result shows it had significant difference at the significance level of 5% for each food products. These results indicate the suitability of Petrifilm EB incubated at 37°C as an alternative to ISO method. The benefit of Petrifilm EB is easy to use, faster time to result, no need to prepare the medium, and no need of confirmation test. The count results of Enterobacteriaceae would be changed depending on the condition of incubation. So it would be important that Petrifilm EB incubation condition should be identical to that of official reference method.

Journal

Japanese Journal of Food Microbiology

Japanese Journal of Food Microbiology 28 (3), 167-174, 2011

Japanese Society of Food Microbiology

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