Protective effects of Aspergillus oryzae NK-fermented grain germ on dextran sulfate sodium-induced colitis in mice.

DOI
  • NOGUCHI Takayasu
    Sagami Laboratory, Wakamoto Pharmaceutical Co., LTD
  • TAKAHASHI Ryoki
    Sagami Laboratory, Wakamoto Pharmaceutical Co., LTD General Medicine and Community Health Science of the Sasayama Medical Center, Hyogo College of Medicine
  • SHIMOYAMA Tadashi
    Department of Gastroenterology, Hirosaki University Graduate School of Medicine
  • OKUDA Masumi
    General Medicine and Community Health Science of the Sasayama Medical Center, Hyogo College of Medicine
  • FUKUDA Yoshihiro
    General Medicine and Community Health Science of the Sasayama Medical Center, Hyogo College of Medicine

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Other Title
  • アスペルギルス・オリゼーNK菌発酵穀物胚芽によるマウスのデキストラン硫酸ナトリウム惹起大腸炎に対する防御効果とその機序

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【Objective】To estimate the protective efficacy of Aspergillus oryzae NK-fermented grain germ (A. oryzae NK-Koji) on inflammatory bowel disease, we examined the effects of the A. oryzae NK-Koji on pathophysiology and colonic mucosal cytokines of dextran sulfate sodium (DSS)-induced colitis in mice.<br>【Materials & Methods】Female Balb/c mice were randomly divided into 4 group (n=6/group). Mice in A. oryzae NK-Koji group were fed purified diet AIN-93M containing 2% or 5% the A. oryzae NK-Koji for 14 days before the induction of colitis and for 6 days thereafter. Colitis was induced by 3% DSS water administration ad libitum for 6 days. Mice in DSS-colitis control groups received the DSS water ad libitum for 6 days after the AIN-93M diet for 14 days. Normal control successively received the AIN-93M. On the end of the experimental period, the pathological findings of colonic tissue and the alteration of colonic mucosal cytokine levels were examined.<br>【Results】Administration of A. oryzae NK-Koji to DSS-ingested mice improved the body weight loss, the anus bleeding, the shortening of the colon length, the histological damage in the colon, and significantly reduced the overproduction of IL-6, IL-1β and TNF-α in the colonic mucosa of DSS-induced colitis in mice. Administration of the A. oryzae NK-Koji brought IFN-γ/IL-10 ratio in the colonic mucosa close to that of the normal control.<br>【Conclusion】A. oryzae NK-Koji demonstrated its protective efficacy on the pathological findings of the colonic tissue and the overproduction of inflammatory cytokines in the colonic mucosa of DSS-induced colitis in mice.

Journal

  • Jomyaku Keicho Eiyo

    Jomyaku Keicho Eiyo 29 (5), 1193-1199, 2014

    Japanese Society for Parenteral and Enteral Nutrition

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