Expression of pancreatic enzyme genes during the early larval stage of Japanese eel Anguilla japonica.
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- KUROKAWA TADAHIDE
- National Research Institute of Aquaculture, Fisheries Research Agency
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- SUZUKI TOHRU
- National Research Institute of Aquaculture, Fisheries Research Agency
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- OHTA HIROMI
- National Research Institute of Aquaculture, Fisheries Research Agency
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- KAGAWA HIROHIKO
- National Research Institute of Aquaculture, Fisheries Research Agency
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- TANAKA HIDEKI
- National Research Institute of Aquaculture, Fisheries Research Agency
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- UNUMA TATSUYA
- National Research Institute of Aquaculture, Fisheries Research Agency
Bibliographic Information
- Other Title
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- Expression of pancreatic enzymes genes during the early larval stage of Japanese eel Anguilla japonica
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Abstract
To reveal the ontogeny of pancreatic exocrine function in the early larval stage of eel, cDNAs encoding major pancreatic enzymes, trypsinogen, amylase and lipase were identified from the Japanese eel Anguilla japonica and their expression pattern in larvae was analyzed. The cloned eel trypsinogen precursor consisted of 224 amino acids and showed 82.2% identity to trypsinogen-2 of winter flounder Pleuronectes americanus. The eel amylase precursor consisted of 512 amino acids and showed 77% identity to winter flounder amylase. Eel pancreatic lipase was composed of 470 amino acids and had 58.3% of identity to human pancreatic lipase. In the eel larvae, mRNA expression of trypsinogen and amylase was first detected at 6 days post-hatching (d.p.h.), and the expression level increased between 7 and 8 d.p.h. In contrast, mRNA expression of lipase was first detected at 8 d.p.h. Eel larvae start to feed actively at 8 d.p.h. Thus, it was indicated that eel pancreas starts to synthesize digestive enzymes at 6 d.p.h. and acquires full function by the onset of exogenous feeding at 8 d.p.h.
Journal
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- Fisheries science
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Fisheries science 68 (4), 736-744, 2002
The Japanese Society of Fisheries Science
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Details 詳細情報について
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- CRID
- 1390001204427918464
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- NII Article ID
- 130003903682
- 10009267431
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- NII Book ID
- AA10993718
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- ISSN
- 14442906
- 09199268
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- NDL BIB ID
- 6267948
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed