The Role of Common Carp (<i>Cyprinus carpio</i>) Muscle Creatine Kinase Isoenzymes at Temperature Acclimation
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- Sun Hsi-Wen
- Graduate Institute of Life Science, National Defense Medical Center Institute of Zoology, Academia Sinica
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- Liu Cheng-Wen
- Institute of Zoology, Academia Sinica
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- Wu Chih-Lu
- Institute of Zoology, Academia Sinica
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- Hui Cho-Fat
- Institute of Zoology, Academia Sinica
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- Wu Jen-Leih
- Institute of Zoology, Academia Sinica
Bibliographic Information
- Other Title
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- The role of common carp(Cyprinus carpio) muscle creatine kinase isoenzymes at temperature acclimation
- role of common carp Cyprinus carpio muscle creatine kinase isoenzymes at temperature acclimation
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Abstract
Creatine kinase plays a key role in the energy metabolism of cells that have fluctuating energy requirements. In vertebrates, the creatine kinase isoenzyme family consists two cytosolic and two mitochondrial forms. Only one M-CK isoform was known until we identified another three carp M-CK isoenzymes. Herein, we examine the effect of temperature changes on the temporal and spatial expressions of these three M-CK isoenzymes. Enzyme-linked immunosorbent assay experimental results indicate that these three M-CK isoforms are expressed at each temperature examined. Consistent with this result, immuno- histochemical staining also showed that these M-CK isoenzymes are co-localized in the same cellular compartment. In addition, the formation of both homo- and heterogeneous complexes among M 1-, M 2, and M3-CK was darified by coimmunoprecipitation and in vitro transcription and translation coupled GST-pull-down assay. We also generated M-CK homo- and heterodimer models to demonstrate the interactions of these monomers. Therefore, we propose that M-CK homo- or heterodimer formation undergoes an exchange according to acclimation to mircoenvironmental temperatures and relative pH values. We think that this MM-CK homo- and heterodimer exchange plays an important role as a temporal energy buffer with a spatial buffering function.
Journal
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- Fisheries science
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Fisheries science 68 (sup2), 1033-1036, 2002
The Japanese Society of Fisheries Science
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Details 詳細情報について
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- CRID
- 1390001204428229504
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- NII Article ID
- 130003903726
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- NII Book ID
- AA10993718
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- NDL BIB ID
- 6422633
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- ISSN
- 09199268
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed