Method not Requiring Mercuric Chloride for the Determination of Activity of 5-Aminolevulinic Acid Dehydratase in Blood of Carp Cyprinus carpio

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  • Nakagawa Hisaki
    Department of Fisheries, Faculty of Agriculture, Kyushu University
  • Sato Tsutomu
    Department of Fisheries, Faculty of Agriculture, Kyushu University
  • Kubo Hirokatsu
    Department of Fisheries, Faculty of Agriculture, Kyushu University

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タイトル別名
  • Method not Requiring Mercuric Chloride for the Determination of Activity of 5-Aminolevulinic Acid Dehydratase in Blood of Carp <i>Cyprinus carpio</i>
  • Method not Requiring Mercuric Chloride

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This study was conducted in order to improve a method for determining the activity of 5-aminolevulinic acid dehydratase (ALA-D) in the blood of carp Cyprinus carpio using the reagent without HgCl2. Seven % TCA solution was used to terminate the enzyme reaction of ALA-D and to removeSH groups which impede the coloring reaction with the Ehrlich reagent. HgCl2 was also removed fromthe Ehrlich reagent. In the case of the modified method which did not use HgCl2, the reaction mixturewas left to stand for 10 min after being at first immediately mixed three times, then, after 10 min andthirdly, after 20min following the addition of the TCA solution to the reaction mixture. When the bloodALA-D activities of carp exposed to various concentrations of lead in water were determined by thepresent method, the levels of ALA-D activities were found to be much the same as the previous methodusing HgCl2.

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