Isolation and Amino Acid Sequence of a Polypeptide Toxin from the Sea Anemone <i>Radianthus crispus</i>
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- Shiomi Kazuo
- Department of Food Science and Technology, Tokyo University of Fisheries
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- Lin Xin-Yu
- Department of Food Science and Technology, Tokyo University of Fisheries
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- Nagashima Yuji
- Department of Food Science and Technology, Tokyo University of Fisheries
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- Ishida Masami
- Department of Food Science and Technology, Tokyo University of Fisheries
Bibliographic Information
- Other Title
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- Isolation and Amino Acid Sequence of a Polypeptide Toxin from the Sea Anemone Radianthus crispus
- Isolation and Amino Acid Sequence of a
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Abstract
The aqueous extract of the sea anemone Radianthus crispus was potently lethal tocrabs but had no toxicity in mice. A polypeptide toxin (named Re I) was easily isolated by gel filtration on Sephadex G-50 and reverse-phase HPLC on TSKgel ODS-80Ts. Its minimum lethal dose against crabs was estimated to be 7.3 μg/kg. The complete amino acid sequence of Rc I was determined by sequence analyses of S-carboxamidomethylated toxin and its enzymatic fragments. Rc I comprises 47 amino acid residues and is characterized by the abundance of Asx, Gly, and half-Cys, the absence of Ala, Met, and Tyr, and the presence of an unusual amino acid, hydroxyproline. As compared with the sequences of the known sea anemone toxins, Rc I is ananalogue of type 1 toxins, showing an especially high homology with Cp I (89%) and Cp II (81%) from the Caribbean sea anemone Condylactis passiflora.
Journal
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- Fisheries science
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Fisheries science 62 (4), 629-633, 1996
The Japanese Society of Fisheries Science
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Details 詳細情報について
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- CRID
- 1390001204428312704
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- NII Article ID
- 130003742050
- 10004867027
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- NII Book ID
- AA10993718
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- NDL BIB ID
- 4014523
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- ISSN
- 09199268
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed