Introduction of pBIN19 Plasmid Gene into the Marine Origin <i>Pseudomonas</i> SC9 Using Electroporation
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- Yoshieda Megumi
- National Research Institute of Aquaculture
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- Nakayama Ichiro
- National Research Institute of Aquaculture
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- Maeda Masachika
- National Research Institute of Aquaculture
Bibliographic Information
- Other Title
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- Introduction of pBIN19 Plasmid Gene into the Marine Origin Pseudomonas SC9 Using Electroporation
- Introduction of pBIN19 Plasmid Gene int
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Abstract
Introduction of new functions to a marine bacterium was aimed at by using a plasmid vector. The plasmid pBIN 19, derived from the broad host range plasmid RK 2, was introduced into marine isolated Pseudomonas strain SC 9 by electroporation. Despite the low transformation efficiency of the bacterial conjugation method reported previously, a transformation efficiency of 1.81×104transformants/μg of pBIN 19 was obtained for the strain SC 9 by electroporation. The introduced pBIN 19was not detected in plasmid form in transformed cell. Southern blot hybrids appeared in genomic DNA under 50 kb using crossed field gel electrophoresis, which indicated introduction and recombination of pBIN 19 into genomic DNA of Pseudomonas SC 9.
Journal
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- Fisheries science
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Fisheries science 61 (6), 898-903, 1995
The Japanese Society of Fisheries Science
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Details 詳細情報について
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- CRID
- 1390001204428880640
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- NII Article ID
- 130003902827
- 10004863984
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- NII Book ID
- AA10993718
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- NDL BIB ID
- 3920555
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- ISSN
- 09199268
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed