Purification and Characterization of Chitinase from the Liver of Japanese Common Squid Todarodes pacificus

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  • Purification and Characterization of Chitinase from the Liver of Japanese Common Squid <i>Todarodes pacificus</i>
  • Purification and Characterization of Ch

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A chitinase (EC 3. 2. 1. 14) was purified from the liver of Japanese common squid Todarodes pacificus by ammonium sulfate fractionation and column chromatographies with Chitopearl Basic BL-03, CM-Toyopearl 650 S, and Bio-Gel HTP. The purified enzyme gave a single protein band on polyacrylamide gel electrophoresis (PAGE), and its molecular weight was estimated tobe 38 kDa by SDS-PAGE. The isoelectric point was 8. 3. The enzyme showed two optimum pHs at 1.5 and 8.5 using glycol chitin as the substrate. The enzyme was stable between pH 4.0 and 6.0 after incubation at 50°C for 10 min. The optimum temperature was 50°C. The chitinase hydrolyzed glycol chitin and colloidal chitin, but not p-nitrophenyl N-acetyl-β-D-glucosaminide and Micrococcus lysodeikticus. The cleavage pattern was investigated using N-acetylchitooligosaccharides (GlcNAcn, n=2 to 6). The enzyme hydrolyzed GlcNAc4 to two molecules of GlcNAc2, GlcNAc5 to GlcNAc2 plus GlcNAc3, and GlcNAc6 to GlcNAc2 plus GlcNAc4 (84%) and two molecules of GlcNAc3 (16%). The substrate specificityof the enzyme was that of endo-type chitinase.

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