cDNA Cloning of Heat-inducible HSP70, a 70.6 kDa Heat Shock Protein, in Japanese Flounder <i>Paralichtys olivaceus</i>
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- Yokoyama Yoshihiro
- Interdisciplinary Research Institute for Biosciences, Mukogawa Women's University
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- Hashimoto Hisashi
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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- Kubota Satoshi
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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- Kinoshita Masato
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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- Toyohara Haruhiko
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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- Sakaguchi Morihiko
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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- Tanaka Masaru
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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- Seikai Tadahisa
- Fisheries Research Station, Graduate School of Agriculture, Kyoto University
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- Kanamori Masao
- Interdisciplinary Research Institute for Biosciences, Mukogawa Women's University
Bibliographic Information
- Other Title
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- cDNA Cloning of Heat-inducible HSP70,a 70.6 kDa Heat Shock Protein,in Japanese Flounder Paralichtys olivaceus
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Abstract
Full-length cDNA for a 70.6 kDa heat-inducible heat shock protein (HSP70), a member of the HSP70 family, was isolated from a cDNA library of cultured cells in early passage originated in Japanese flounder embryos (JFE cells). It has a single ORF of 1920 bp that encodes a protein of 70.6 kDa. Japanese flounder HSP70 contains an EEVD peptide motif at C-terminal end which is a common feature of the cytosolic HSP70 family. Japanese flounder HSP70 is 86.0%, 83.8%, and 83.6% identical in primary structure to chinook salmon HSP70, bovine HSP70, and human HSP70, respectively. Japanese flounder HSP70 is also 83.6% identical to Japanese flounder HSC71. These results suggest that the amino acid sequence of the cytosolic HSP70 family, HSP70 and HSC71, has been highly conserved among vertebrates. Northern blot analysis showed that HSP70 mRNA was induced with heat shock treatment in the JFE cells in early passage.
Journal
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- Fisheries science
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Fisheries science 64 (6), 964-968, 1998
The Japanese Society of Fisheries Science
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Details 詳細情報について
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- CRID
- 1390001204430023424
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- NII Article ID
- 130003903289
- 10004877040
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- NII Book ID
- AA10993718
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- NDL BIB ID
- 107216
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- ISSN
- 09199268
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed