cDNA Cloning of Tropomyosin from the Anterior Byssus Retractor Muscle of Mussel and Its Structural Integrity from the Deduced Amino Acid Sequence
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- Iwasaki Keiko
- Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- Kikuchi Kiyoshi
- Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- Funabara Daisuke
- Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- Watabe Shugo
- Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
書誌事項
- タイトル別名
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- c DNA Cloning of Tropomyosin from the A
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A cDNA clone encoding tropomyosin was isolated from the anterior byssus retractor muscle (ABRM) of the mussel Mytilus edulis galloprovincialis. It contained in total 1, 123 nucleotides (nt) with 5' non-coding 89 nt, 3' non-coding 182 nt and coding 852 nt for 284 amino acid residues, a size typical of muscle tropomyosin. Genomic DNA digests with EcoRI, EcoRV and HindIII all exhibited three bands when hybridized with a HaeIII DNA fragment of tropomyosin cDNA, suggesting that the mussel has multiple genes encoding muscle tropomyosin and related proteins. The mRNAs encoding ABRM tropomyosin were most abundant in muscle tissues from byssus retractor and adductor muscles. Considerably large amounts were also observed with gill and inner mantle, whereas traces were found in outer mantle and foot. In the deduced amino acid sequence of ABRM tropomyosin, the N-terminal MDAIKKKMV was well conserved, which has been reported to be involved in head-to-tail polymerization of the tropomyosin molecules. Our tropomyosin was also suggested to have a coiled-coil structure composed of two α-helices that showed the heptad repeats (a-b-c-d-e-f-g)n where a and d tended to be occupied by nonpolar residues with further 28-amino acid repeat zones.
収録刊行物
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- Fisheries science
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Fisheries science 63 (5), 731-734, 1997
公益社団法人 日本水産学会
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詳細情報 詳細情報について
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- CRID
- 1390001204430305536
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- NII論文ID
- 130003903103
- 10004871450
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- NII書誌ID
- AA10993718
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- COI
- 1:CAS:528:DyaK2sXntVGrt7w%3D
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- NDL書誌ID
- 4326625
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- ISSN
- 09199268
- http://id.crossref.org/issn/09199268
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- CiNii Articles
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- 使用不可