Changes of the Solubility and ATPase Activity of Carp Myofibrils during Frozen Storage at Different Temperatures
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- Yoshikawa Kuniaki
- Central Research Laboratory, Taiyo Kagaku Co., Ltd.
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- Inoue Norio
- Faculty of Fisheries, Hokkaido University
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- Kawai Yuji
- Faculty of Fisheries, Hokkaido University
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- Shinano Haruo
- Faculty of Fisheries, Hokkaido University
書誌事項
- タイトル別名
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- Changes of the Solubility and ATPase Ac
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抄録
The effect of the unfrozen phase under frozen storage conditions on the solubilty and ATPase activity of carp myofibrils was compared. Myofibrillar samples were suspended in 0.1M KCl solution containing 5 and 20mM Tris-maleate buffer (pH 7.2), and in 0.1M KCl solution without a buffer. Sampleswere stored at threedifferent temperatures, -4, -11, and -26°C. When the decrease in ATPase activitywas larger than that of the soluble protein during storage above and nearthe eutectic point of KCl (at-4 and -11°C), and in the presence of 20mM Tris-maleate buffer, it is assumed that the myofibrils are greatly affected by a small amountoof concentrated KCl solution. However, in the absence of a buffer, Where the opposite occurs in both indices, the denaturation of myofibrils is thought to be caused largely by the moving closer together and maldistribution of protein molecules. This is possible to explain in terms of the amount of unfrozen solution being sufficient for maldistribution, but insufficient for inactivation. On the other hand, when stored below the eutectic point of KCl (at-26°C), the decrease in both indices was smaller than that seen in samples stored at -4 and -11°C.This could be ascribed to the fact that myofibrillar protein and crystallized KCl are dispersed uniformly due to the small amount of unfrozen solution, insufficient for bothmaldistribution and inactivation. The surface hydrophobicity was increased in the early stages of storage at both-4°C and-11°C.
収録刊行物
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- Fisheries science
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Fisheries science 61 (5), 804-812, 1995
公益社団法人 日本水産学会
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詳細情報 詳細情報について
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- CRID
- 1390001204430349824
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- NII論文ID
- 130003902790
- 10004863612
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- NII書誌ID
- AA10993718
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- NDL書誌ID
- 3641425
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- ISSN
- 09199268
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可