Isolation and Characterization of Troponin from Abdominal Muscle of Prawn Penaeus japonicus

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  • Ojima Takao
    Department of Chemistry, Faculty of Fisheries, Hokkaido University
  • Toyoguchi Toshiyuki
    Department of Chemistry, Faculty of Fisheries, Hokkaido University Environment Conrol Center Co., Ltd.
  • Nishita Kiyoyoshi
    Department of Chemistry, Faculty of Fisheries, Hokkaido University

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  • Isolation and Characterization of Troponin from Abdominal Muscle of Prawn <i>Penaeus japonicus</i>
  • Isolation and Characterization of Tropo

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Troponin, which confers high Ca2+-sensitivity to rabbit reconstituted actomyosin, was isolated from the abdominal muscle of prawn Penaeus japonicus. SDS-polyacrylamide gel electrophoresis showed that the prawn troponin consists of three components of Mr 17, 000, 30, 000-32, 000 (doublet), and 42, 000 in a molar ratio of 1:1:1, but Mr 52, 000-59, 000 component, which had been reported to be a troponin-T-like protein in arthropod troponin, was not found. The three components were separated from each other by DEAE-Toyopearl column chromatography in the presence of 6M urea, and assigned as troponin-C, troponin-I, and troponin-T in increasing order of molecular weight. These features were basically identical with those of American lobster troponin of our previous study (Nishita and Ojima, J. Biochem., 108, 677-683 (1990)).

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