Development of an Enzyme Sensor Using Multi-enzyme System Immobilized in β-Chitin Membrane
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- Ohashi Eiji
- Central Research Laboratory, Nippon Suisan Kaisha, Ltd.
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- Karube Isao
- Research Center for Advanced Science and Technology, University of Tokyo
書誌事項
- タイトル別名
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- Development of an Enzyme Sensor Using Multi-enzyme System Immobilized in β-Chitin Membrane
- Development of an Enzyme Sensor Using M
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A multi-enzyme system was immobilized in a β-chitin membrane. β-Chitin was obtained from common squid pens and glutamate pyruvate transaminase and pyruvate oxidase were immobilized simultaneously in the β-chitin membrane. The immobilization was achieved by filtration of the chitin suspension containing enzymes without any chemical reagent or pH treatment. An enzyme electrode for the measurement of L-alanine was assembled with the β-chitin membrane and an oxygenelectrode, and enzyme activities were evaluated. A control membrane was prepared using a cross-linking method with enzymes, bovine serum albumin and glutaraldehyde. The electrode response of the β-chitin membrane was obtained immediately after the sample injection and leveled off in 60 s. In contrast, the control membrane showed a slower response. The maximum response of the β-chitin embrane electrode was obtained at 38°C and pH 7.1. Linearity of the calibration curve was obtained up to 30mM in the batch system and 200mM in the flow system. These results suggest that β-chitin is a useful supporting material for enzyme immobilization and applicable to enzyme electrodes.
収録刊行物
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- Fisheries science
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Fisheries science 61 (5), 856-859, 1995
公益社団法人 日本水産学会
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詳細情報 詳細情報について
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- CRID
- 1390001204430354560
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- NII論文ID
- 130003902795
- 10004863797
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- NII書誌ID
- AA10993718
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- NDL書誌ID
- 3642540
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- ISSN
- 09199268
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可