Genetic and cytological analysis of a sporulation-deficient (sporeless) mutant of <i>Pleurotus cornucopiae</i> var. <i>citrinopileatus</i> induced by UV irradiation

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  • YONEYAMA Shozo
    Hokkaido Research Organization Forest Products Research Institute
  • ANDO Natsumi
    Fungus/Mushroom Resource and Research Center, Faculty of Agriculture, Tottori University
  • AZUMA Tomonori
    Hokkaido Research Organization Forest Products Research Institute
  • SATO Mayumi
    Hokkaido Research Organization Forest Products Research Institute
  • USHIJIMA Shuji
    Fungus/Mushroom Resource and Research Center, Faculty of Agriculture, Tottori University
  • MATSUMOTO Teruyuki
    Fungus/Mushroom Resource and Research Center, Faculty of Agriculture, Tottori University

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Other Title
  • タモギタケ胞子欠損性変異体に関する遺伝学的および細胞学的解析
  • タモギタケ ホウシ ケッソンセイ ヘンイタイ ニ カンスル イデンガクテキ オヨビ サイボウガクテキ カイセキ

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<p>　A sporulation-deficient (sporeless) mutant of Pleurotus cornucopiae var. citorinopileatus, induced by UV irradiation, was cytogenetically and genetically characterized. The sporeless mutant formed less than 0.1％ of the basidiospores produced by wild-type P. cornucopiae. The sporulation-deficient phenotype was controlled by a single dominant gene. Basidia and basidiospores on the gill surface of the wild-type strain exhibited consistent shape and size. By contrast, the few basidiospores produced by the sporeless mutant were highly variable in length, with some very long spores observed, and spores were often wrinkled and shrunk. Basidia with fewer than four sterigmata were frequently observed. HCl-Giemsa staining revealed aberrant nuclear characteristics in the sporeless mutant, such as nuclear localization in the basidium, abortive basidiospores with no nucleus, and basidia without tetrad sterigma formation during sporulation. This suggested that genes involved in sporulation and basidispore maturation were adversely affected and that the sporulation deficiency was caused by atypical migration of nuclei.</p>

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