Short-term hypergravity does not affect protein-ubiquitination and proliferation in rat L6 myoblastic cells

DOI Web Site PubMed 被引用文献2件 参考文献16件 オープンアクセス
  • Hirasaka Katsuya
    Department of Nutrition, The University of Tokushima School of Medicine, Tokushima 770-8503, Japan
  • Nikawa Takeshi
    Department of Nutrition, The University of Tokushima School of Medicine, Tokushima 770-8503, Japan
  • Asanoma Yuki
    Department of Nutrition, The University of Tokushima School of Medicine, Tokushima 770-8503, Japan
  • Furochi Harumi
    Department of Nutrition, The University of Tokushima School of Medicine, Tokushima 770-8503, Japan
  • Onishi Yuko
    Department of Nutrition, The University of Tokushima School of Medicine, Tokushima 770-8503, Japan
  • Ogawa Takayuki
    Department of Orthopaedics, The University of Tokushima School of Medicine, Tokushima 770-8503, Japan
  • Suzue Naoto
    Department of Orthopaedics, The University of Tokushima School of Medicine, Tokushima 770-8503, Japan
  • Oarada Motoko
    Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba 260-8673, Japan
  • Shimazu Toru
    Japan Space Forum, Tokyo 105-0013, Japan
  • Kishi Kyoichi
    Department of Nutrition, The University of Tokushima School of Medicine, Tokushima 770-8503, Japan

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説明

We previously reported that spaceflight (STS-90) and tail-suspension stimulated muscle protein ubiquitination and accumulated the degradation fragments. However, in space experiments the side-effects of hypergravity on samples are inevitable during the launch of a space shuttle into space or the reentry. To examine whether hypergravity also caused protein-ubiquitination in skeletal muscle cells, we exposed rat myoblastic L6 cells to various hypergravity conditions. Immunoblot analysis showed that the centrifugation at 2, 3, 30 or 100G for 10 min did not increase the amount of ubiquitinated proteins in L6 cells, whereas the centrifugation at 100G for 1 or 2 hrs significantly induced the protein-ubiquitination. In contrast, heat shock protein 70 (HSP70), another stress-responsive protein, in L6 cells was accumulated only by centrifugation at 100G for more than 10 min. Short-term (10 min) hypergravity including 3 or 100 G did not affect the proliferation and morphological changes in L6 cells. Our present results suggest that the ubiquitination of muscle proteins is less sensitive to hypergravity than the induction of HSP70, and that the effect of hypergravity on protein-ubiquitination and proliferation of skeletal muscle cells may be negligible, as far as its duration is short-term.

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