Preparation of Cation-Exchange Particle Designed for High-Speed Collection of Proteins by Radiation-Induced Graft Polymerization

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  • SEKIYA Yuta
    Department of Applied Chemistry and Biotechnology, Chiba University
  • SHIMODA Yuichi
    Department of Applied Chemistry and Biotechnology, Chiba University
  • UMENO Daisuke
    Department of Applied Chemistry and Biotechnology, Chiba University
  • SAITO Kyoichi
    Department of Applied Chemistry and Biotechnology, Chiba University
  • FURUMOTO Goro
    Microza & Water Processing Division, Asahi Kasei Chemicals Corporation
  • SHIRATAKI Hironobu
    Microza & Water Processing Division, Asahi Kasei Chemicals Corporation
  • SHINOHARA Naoyuki
    Microza & Water Processing Division, Asahi Kasei Chemicals Corporation
  • KUBOTA Noboru
    Microza & Water Processing Division, Asahi Kasei Chemicals Corporation

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Other Title
  • 放射線グラフト重合法を用いたタンパク質高速回収用カチオン交換粒子の作製
  • ホウシャセン グラフト ジュウゴウホウ オ モチイタ タンパクシツ コウソク カイシュウヨウ カチオン コウカン リュウシ ノ サクセイ

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Abstract

A cation-exchange polymer brush was immobilized onto a polyethylene-based particle with an average diameter of 35 μm by radiation-induced graft polymerization of glycidyl methacrylate and subsequent sulfonation with sodium sulfite. A lysozyme solution was forced to flow through a bed (height 2 cm, cross-sectional area 0.61 cm2) charged with the resultant cation-exchange particles at a space velocity ranging from 500 to 2300 h-1. From a viewpoint of equilibrium binding capacity and elution percentage of lysozyme, the dose of electron beam and the degree of GMA grafting were optimized to be 200 kGy and 100%, respectively. The bed exhibited a constant dynamic binding capacity of lysozyme 14 mg⁄mL irrespective of space velocity due to negligible diffusional mass-transfer resistance.

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