Separation of γ-Aminobutyric Acid from Enzyme Reaction Solution Using Electrodialysis and Diffusion Dialysis

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  • TAKAHASHI Hiroshi
    Department of Engineering in Applied Chemistry, Faculty of Engineering and Resource Science, Akita University
  • KAMEYAMA Tomoaki
    Department of Engineering in Applied Chemistry, Faculty of Engineering and Resource Science, Akita University
  • KIKUCHI Ken-Ichi
    Department of Engineering in Applied Chemistry, Faculty of Engineering and Resource Science, Akita University

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Other Title
  • イオン交換膜を用いた電気透析法ならびに拡散透析法による酵素反応溶液からのγ-アミノ酪酸の分離
  • イオン コウカン マク オ モチイタ デンキ トウセキホウ ナラビニ カクサン トウセキホウ ニ ヨル コウソ ハンノウ ヨウエキ カラ ノ ガンマ アミノラクサン ノ ブンリ
  • Separation of ^|^gamma;-Aminobutyric Acid from Enzyme Reaction Solution Using Electrodialysis and Diffusion Dialysis

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Abstract

Membrane permeation characteristics of γ-aminobutyric (GABA), glutamic, and aspartic acids were examined in electrodialysis and/or diffusion dialysis. In the electrodialysis experiments at pH 3, γ-aminobutyric acid permeated through a cation-exchange membrane, but glutamic acid did not. In the experiment of diffusion dialysis of γ-aminobutyric, aspartic, and glutamic acids, the fluxes of those amino acids were affected by electrolytes species and their concentration in the Strip solution. Moreover, the diffusion-dialysis technique was applied to separation of amino acids from the resultant solution of the reaction of glutamic acid using rice bran. The fluxes of amino acids were increased with an increase in HCl concentration of the Strip solution, and these values were in the order of amino acids γ-aminobutyric acid>aspartic acid>glutamic acid. These results suggest that diffusion dialysis with a cation-exchange membrane is applicable to the selective separation of γ-aminobutyric acid from the enzyme reaction solution with glutamic acid and rice bran.<br>

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