Assessment of the Genotoxicity of 1,2‐Dichloropropane and Dichloromethane after Individual and Co‐exposure by Inhalation in Mice
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- Suzuki Tetsuya
- Division of Health Effects Research, National Institute of Occupational Safety and Health
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- Yanagiba Yukie
- Division of Health Effects Research, National Institute of Occupational Safety and Health
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- Suda Megumi
- Division of Health Effects Research, National Institute of Occupational Safety and Health
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- Wang Rui-Sheng
- Division of Health Effects Research, National Institute of Occupational Safety and Health
書誌事項
- タイトル別名
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- Assessment of the Genotoxicity of 1,2-Dichloropropane and Dichloromethane after Individual and Co-exposure by Inhalation in Mice
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説明
Objective: Occurrence of cholangiocarcinoma was recently reported at a high incidence rate among the employees working for an offset printing company in Osaka, Japan. 1,2-Dichloropropane (1,2-DCP) and dichloromethane (DCM) are suspected to be the causes of the cancer, as they had been used as ink cleaners in large amounts. However, it is not clear whether these chlorinated organic solvents played a role in the occurrence of cholangiocarcinoma or why the incidence rate is so high among the workers in this industry. To provide possible evidence for this severe occupational problem, we investigated the genotoxic effects of 1,2-DCP and DCM. Methods: Male B6C3F1 and gpt Delta C57BL/6J mice were exposed by inhalation to the individual solvents or both solvents at multiple concentrations including the levels that were possibly present in the workplaces. The genotoxicity was analyzed by Pig-a gene mutation and micronuclei assays in peripheral blood and gpt mutation and comet assays in the livers of mice after repeated inhalation of 1,2-DCP or/and DCM. Results: The Pig-a mutant frequencies and micronuclei incidences were not significantly increased by exposure of either 1,2-DCP or/and DCM at any concentration, suggesting there was no genotoxic potential in bone marrow for both solvents. In the liver, DNA damage, as measured by the comet assay, was dose dependently increased by 1,2-DCP but not by DCM. The gpt mutant frequency was 2.6-fold that of the controls in the co-exposure group. Conclusions: These results indicate that 1,2-DCP showed stronger genotoxicity in the liver and that the genotoxic effects were greatly enhanced by simultaneous exposure to DCM.(J Occup Health 2014; 56: 205-214)
収録刊行物
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- journal of Occupational Health
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journal of Occupational Health 56 (3), 205-214, 2014
公益社団法人 日本産業衛生学会
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詳細情報 詳細情報について
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- CRID
- 1390001204455797376
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- NII論文ID
- 130004447651
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- NII書誌ID
- AA11090645
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- COI
- 1:CAS:528:DC%2BC2cXht1KmtbzM
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- ISSN
- 13489585
- 13419145
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- NDL書誌ID
- 025490684
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- PubMed
- 24739373
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可