Histopathological Study of Matrix Mineralization by Osteoblastic-like and Odontoblastic-like Cells in Diffusion Chamber

  • Rodriguez Andrea Paola
    Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
  • Borkosky Silvia
    Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
  • Han Phuu Pwint
    Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
  • Tsujigiwa Hidetsugu
    Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
  • Takagi Tohru
    Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
  • Gulsan Ara Sathi
    Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
  • Lee You-Jin
    Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
  • Katase Naoki
    Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
  • Okauchi Mika
    Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
  • Nakano Keisuke
    Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
  • Xiao Jing
    Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Dalian Medical University, Faculty of Dentistry

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Abstract

Bone and dentin resemble each other in composition and mechanism of formation and mineralization. Preliminary analysis of osteoblastic and odontoblastic cells before using them in tissue engineering is mandatory. Thus, in order to evaluate the process of calcification, we evaluated osteoblastic-like (KUSA/A1cells) and odontoblastic-like (MDPC-23 cells) cells seeded in cell culture and in intraperitoneal diffusion chamber. Our results indicated that KUSA/A1 cells differentiated into osteoblasts-like cells and induced bone tissue inside the chamber. Whereas, MDPC-23 cells showed odontoblastic phenotype without ability to induce dentin formation, suggesting that MDPC-23 cells are special cells, which lost the capacity to induce mineralized dentin matrix after long period of time. This study showed the significance of basic information of calcification process by osteoblastic-like and odontoblastic-like cells before using them in tissue engineering.

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