Enhanced Osteogenic and Angiogenic-Related Gene Expression of Human Dental Stem Cells on Biphasic Calcium Phosphate Scaffold Treated with Vascular Endothelial Growth Factor: Part I

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  • Enezei Hamid Hammad
    Department of Oral & Maxillofacial Surgery, College of Dentistry, University of Anbar Department of Oral & Maxillofacial Surgery, School of Dental Science, Universiti Sains Malaysia
  • Ahmad Azlina
    Department of Biochemistry, School of Dental Science, Universiti Sains Malaysia
  • Khamis Mohd Fadhli
    Department of Oral Biology and Forensic Dentistry Unit, School of Dental Science, Universiti Sains Malaysia
  • Suzuki Junji
    Department of Oral Pathology, School of Dentistry, Aichi Gakuin University
  • Sugita Yoshihiko
    Department of Oral Pathology, School of Dentistry, Aichi Gakuin University
  • Maeda Hatsuhiko
    Department of Oral Pathology, School of Dentistry, Aichi Gakuin University
  • Alshehadat Saaid
    Department of Preventive and Restorative Dentistry, College of Dental Medicine, University of Sharjah
  • Razak Noor Hayati Abdul
    Department of Oral & Maxillofacial Surgery, School of Dental Science, Universiti Sains Malaysia
  • Abbas Salah Khalaf
    Department of Prosthodontic Dentistry, College of Dentistry, University of Anbar
  • Qabbani Ali Al
    Department of Craniofacial Health Sciences, College of Dental Medicine, University of Sharjah
  • Alam Mohammad Khursheed
    Orthodontic Department, College of Dentistry, Aljouf University

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  • Enhanced Osteogenic and Angiogenic-Related Gene Expression of Human Dental Stem Cells on Biphasic Calcium Phosphate Scaffold Treated with Vascular Endothelial Growth Factor(Part 1)

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<p>To study the effectiveness of vascular endothelial growth factor (VEGF) added with biphasic calcium phosphate (BCP) on the expression of osteogenesis and angiogenesis-related gene in dental stem cells (DSCs). The cells were treated with three different modalities; BCP group, VEGF group, and VEGF-added-BCP. The optimal BCP and VEGF concentrations were determined. The cells were harvested at four different time intervals (day 3, day 7, day 10 and day 14) and were subjected to RNA isolation. Osteogenesis and angiogenesis-regulated genes was amplified using reverse transcriptase-PCR (RT-PCR). The RT-PCR products were then electrophoresed. The gel images were captured using Image Analyser AlphaEaseFC™. Angiogenesis and osteogenesis genes were clearly expressed in DSCs in response to treatments with 75mg/ml BCP and 5ng/ml VEGF. Angiogenesis gene VEGF was highly expressed by VEGF treatment group but showed some changes when added with BCP. Osteogenesis genes (BMP-2 and OPN) were positively affected by both BCP and VEGF. Some genes were expressed at an earlier time interval compared to the other genes depending on the type of treatments. BCP treatment induced high expression of initial-regulated osteogenesis genes (BMP-2 and OPN). Combination of BCP and VEGF modality on DSCs was suggested to initiate osteogenesis and angiogenesis-related gene expressions earlier than the other modalities. In our design, uncontrolled release of VEGF protein showed inhibition of BMP-2 at mRNA level in VEGF-BCP combined group.</p>

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