Investigation for Effects of Cyclical Dynamic Compression on Matrix Metabolite and Mechanical Properties of Chondrocytes Cultured in Alginate
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- Zhou Wu
- Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
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- Liu Guohui
- Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
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- Yang Shuhua
- Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
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- Ye Shunan
- Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
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Articular cartilage is suffered from high mechanical loads under normal physiological conditions and articular chondrocytes regulate the composition of peri-cellular matrix (PCM), in response to mechanical signals. This study aims to investigate influence of cyclical dynamic compression on matrix metabolite and mechanical properties of chondrocytes cultured in alginate. Two months old New Zealand white rabbits were sacrificed by air embolism. Chondrocytes were taken from knee joints under aseptic conditions and cultured in alginate. The suspension was gelled in a shape of disc in CaCl2 solution and processed for intermittent compression by using Flexcell-5000 compression system kept under an uncompressed condition as a control. The gene regulation of aggrecan (Agg), collagen α! (Col-2), matrix metallo-proteinases 13 (MMP-13) and collagen X (Col-10) were determined by reverse transcription polymerase chain reaction (RT-PCR) at 7th, 14th and 21st day post loading. The alteration in mechanical properties of chondrocytes induced by compression was evaluated using micropipette aspiration technique combined with a half-space model. A significant up-regulation was observed in gene expression of Agg and Col-2 on 7th day or MMP-13 and Col-10 on 14th day respectively after loading of compression. In response to compression, chondrocytes exhibited viscoelastic solid creep behavior. Young’s modulus almost approximated to control group (0.65±0.42kPa). However, viscoelastic properties decreased gradually along with intermittent compression. Viscoelastic properties in experimental 21st day, including equilibrium modulus (E∞), the instantaneous modulus (E0) and apparent viscosity (μ) were significantly lower than experimental 7th day and 14th day (P<0.001), no significant difference was found between experimental 7th day and 14th day (P >0.05). In conclusion, chondrocytes cultured in alginate are stable of mechanical properties and suitable to construct seed cell of tissue engineering, and can keep phenotype and mechanical properties.
収録刊行物
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- Journal of Hard Tissue Biology
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Journal of Hard Tissue Biology 25 (4), 351-356, 2016
硬組織再生生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001204458208640
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- NII論文ID
- 130005249140
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- NII書誌ID
- AA11074332
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- ISSN
- 1880828X
- 13417649
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- NDL書誌ID
- 027698835
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可