Requirement for JNK and ERK Activation in BMP-2/BMP-7 -Induced Osteogenesis of Human Periodontal Ligament Cells

DOI Web Site 参考文献27件
  • Tezen Chikara
    Department of Endodontics and Clinical Cariology, Tokyo Dental College Oral Health Science Center hrc7, Tokyo Dental College
  • Ochiai Hiromi
    Department of Biochemistry, Tokyo Dental College Oral Health Science Center hrc8, Tokyo Dental College
  • Aida Natsuko
    Department of Endodontics and Clinical Cariology, Tokyo Dental College Oral Health Science Center hrc7, Tokyo Dental College
  • Okada Shoko
    Department of Biochemistry, Tokyo Dental College
  • Saito Akiko
    Department of Biochemistry, Tokyo Dental College
  • Azuma Toshifumi
    Department of Biochemistry, Tokyo Dental College Oral Health Science Center hrc7, Tokyo Dental College Oral Health Science Center hrc8, Tokyo Dental College

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The BMP-2/BMP-7 heterodimer (BMP-2/-7) is a potent osteogenic inducer. It is well established that BMP-2/-7 activates mitogen activated protein kinases (MAPKs) in some cells. It is also widely accepted that MAPK signaling cascades play a crucial role in the immediate osteogenic response to BMPs. However, the precise mechanism and role of the MAPK signaling network in osteogenesis is still controversial since there are many kinds of BMP receptors (BMPR) whose signal transduction is very complex and whose signals differ from cell to cell. We investigated whether BMP-2/-7 regulates osteogenic gene expression in human periodontal ligament (HPDL) cells, and how MAPKs might be involved in this process, by treatment of HPDL cells with BMP-2/-7 with or without MAPK inhibitors. We found that the Jun-N-terminal kinase (JNK) is essential for the expression of almost all osteogenic marker genes. Inhibition of extracellular signal-regulated kinase (ERK) using inhibitor had no effect on the expression of early osteogenic marker genes, whereas it significantly down-regulated the expression of late osteogenic marker genes such as Bone sialopotein and Osteocalcin. These results indicate that MAPKs play a crucial role in osteogenesis of HPDL cells.

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