-
- Iwai Satoshi
- Departments of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo
-
- Kuyama Kayo
- Department of Oral Pathology, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
-
- Kuboyama Noboru
- Department of Pharmacology, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
-
- Takiguchi Shimpei
- Departments of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
-
- Ogura Naomi
- Departments of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
-
- Yamamoto Hirotsugu
- Department of Oral Pathology, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
-
- Kondoh Toshirou
- Departments of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
この論文をさがす
抄録
The objective of this study was to investigate the osteogenic potential of human dental follicle cells (hDFC) in vivo. hDFC were isolated from dental follicle tissue by enzymatic digestion and cultured in growth medium (GM). hDFC were grown in a three-dimensional (3D) culture using gelatin sponges in osteogenic induction medium (OIM) or GM. The cells were transplanted onto calvaria bone of immunodeficient rats (n = 3). Hematoxylin and eosin (H-E) staining and immunohistochemistry were performed, and bone formation was analyzed with micro-computed tomography (micro-CT). H-E staining showed newly formed bone after transplantation of hDFC grown in a 3D culture in OIM. Immunohistochemistry showed BMP-2, Runx2, and Osterix expression in the hDFC transplantation site during the early stage of bone formation. Moreover, micro-CT showed that the transplanted hDFC that were 3D-cultured in OIM promoted good bone quality and bone volume compared to the other two groups. Thus, human dental follicles may be a potentially useful cell source for regenerative therapy.
収録刊行物
-
- Journal of Hard Tissue Biology
-
Journal of Hard Tissue Biology 22 (1), 95-104, 2013
硬組織再生生物学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390001204458646912
-
- NII論文ID
- 130004848416
-
- NII書誌ID
- AA11074332
-
- COI
- 1:CAS:528:DC%2BC3sXms1Kgt7Y%3D
-
- ISSN
- 1880828X
- 13417649
-
- NDL書誌ID
- 024429725
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可