The Pro-Healing Effect of Protamine-Hydrolysate Peptides on Skin Wounds Involves TGF-β/Smad Signaling
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- K. Bhawal Ujjal
- Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo
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- Lee Hye-jin
- Department of Preventive and Public Health Dentistry, School of Dentistry, Chonnam National University
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- Uchida Ryoichiro
- Department of Dental Materials, Nihon University School of Dentistry at Matsudo
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- Okumura Shigetoshi
- Rohto Pharmaceutical Co., Ltd
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- Harayama Shuichiro
- Harayama Dental Office
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- Eguchi Yawara
- Department of Orthopaedic Surgery, Chiba University School of Medicine
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- Fukumoto Masahiko
- Department of Laboratory Medicine, Nihon University School of Dentistry at Matsudo
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- Kuboyama Noboru
- Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
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Abstract
This study characterized the molecular mechanisms of the effects of protamine-hydrolysate peptides (p-h peptides) on skin wound healing in rats by analyzing the transforming growth factor (TGF)-β signaling pathway. TGF-β was expressed in experimentally wounded skin tissues in fibroblasts and in keratinocytes. In p-h peptides-treated animals, the skin wounds exhibited an increased expression of TGF-β and of TGF-β target genes compared with control saline-treated skin wounds. Treatment with p-h peptides accelerated wound epithelialization and induced protein expression of TGF-β, CTGF and VEGF. The expression of tumor necrosis factor (TNF)-α was decreased in fibroblasts of p-h peptides-treated skin wounds. In addition, treatment with p-h peptides significantly enhanced the phosphorylation of Smad3 and Smad4 in fibroblasts and also elevated the phosphorylation of Stat3 in skin wound tissues. In conclusion, treatment with p-h peptides activated the Smad-dependent TGF-β signaling pathway, enhanced the differentiation of myofibroblasts and accelerated skin wound closure.
Journal
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- Journal of Hard Tissue Biology
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Journal of Hard Tissue Biology 24 (1), 91-98, 2015
THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY
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Details 詳細情報について
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- CRID
- 1390001204459240704
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- NII Article ID
- 130004848449
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- NII Book ID
- AA11074332
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- ISSN
- 1880828X
- 13417649
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- NDL BIB ID
- 026059046
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed