Development of an Easy and High-Throughput Cell Assay System with a Culture Chip and an Assay Chip
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- Sugiura Kanako
- Nagoya University
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- Kaji Noritada
- Nagoya University
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- Okamoto Yukihiro
- Nagoya University
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- Tokeshi Manabu
- Nagoya University
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- Baba Yoshinobu
- Nagoya University
Bibliographic Information
- Other Title
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- 培養チップとアッセイチップを組み合わせた簡便なハイスループット細胞アッセイシステムの開発
- バイヨウ チップ ト アッセイチップ オ クミアワセタ カンベン ナ ハイスループット サイボウ アッセイ システム ノ カイハツ
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Abstract
High throughput cell assay is significantly important in drug screening, assessment of toxicity etc. Cell assay with a microchip is one of the candidates for high throughput cell assay. However, reported cell assay system with the microchip requires expensive apparatus for refluxing medium and investigation of optimum experimental condition for steady data. For an inexpensive, easy and high throughput cell assay, we introduce a new cell assay system combined with a culture chip and an assay chip made of poly(dimethyl siloxane). Cell culture chips enabled cell to proliferate along the microchannel without refluxing medium and permitted to prepare cell patterning easily. Also, assay chips formed concentration gradient inside the chip and allowed the cell assay with different concentrations of drug at the same time. Thus, our developed cell assay system can overcome the problems of the present cell assay and would promote the drug discovery, assessment of toxicity etc.
Journal
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- IEEJ Transactions on Sensors and Micromachines
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IEEJ Transactions on Sensors and Micromachines 130 (10), 471-475, 2010
The Institute of Electrical Engineers of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390001204460229888
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- NII Article ID
- 10026694525
- 130000410305
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- NII Book ID
- AN1052634X
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- ISSN
- 13475525
- 13418939
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- NDL BIB ID
- 10833217
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed