Development of a Microreactor for PCR
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- Kondo Seiji
- OLYMPUS OPTICAL. CO., Ltd.
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- Morimoto Nobuhiko
- OLYMPUS OPTICAL. CO., Ltd.
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- Hori Kunio
- OLYMPUS OPTICAL. CO., Ltd.
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- Shinohara Etsuo
- OLYMPUS OPTICAL. CO., Ltd.
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- Kano Kyoichi
- OLYMPUS OPTICAL. CO., Ltd.
Bibliographic Information
- Other Title
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- PCRのためのマイクロリアクターの開発
- PCR ノ タメ ノ マイクロリアクター ノ カイハツ
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Abstract
We have developed a microreactor for Polymerase Chain Reaction (PCR) by means of microfabrication technologies. The microreactor has been constructed to amplify DNAs, which was much faster than a conventional apparatus. Its size was 30×16mm, in which a reaction chamber with 10mm square and 300 μm depth was constracted. It was found that this microreactor requires only 20 minutes to perform 40 cycles of a shuttle PCR, 3 times faster than conventional apparatus.
Journal
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- IEEJ Transactions on Sensors and Micromachines
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IEEJ Transactions on Sensors and Micromachines 119 (10), 448-453, 1999
The Institute of Electrical Engineers of Japan
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Details 詳細情報について
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- CRID
- 1390001204461529344
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- NII Article ID
- 10004834259
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- NII Book ID
- AN1052634X
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- ISSN
- 13475525
- 13418939
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- NDL BIB ID
- 4866308
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed