電気泳動用石英ガラス製マイクロチップの開発

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タイトル別名
  • Microfabricated Chips for Capillary Electrophoresis on Quartz Glass Substrates
  • デンキ エイドウヨウ セキエイ ガラスセイ マイクロチップ ノ カイハツ

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抄録

Microchips for capillary electrophoresis have been fabricated in small size on quartz glass substrates. Microchannels with smooth sidewalls are etched by applying adopted etching condition. To fabricate quartz glass chips, we applied a novel bonding method with dilute hydrofluoric acid solution. Using fabricated microchips, we show that they permit to perform quantitative sample injection (several 10pl or less), effective separation (the number of theoretical plates is over 2, 500) and high speed analysis (_??_30 sec). In order to improve the detection sensotivity of UV absorbance method, we have developped the chips which have optical slit, and confirmed that the absorbance unit was improved about ten times.

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