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- 安藤 毅
- 埼玉大学大学院理工学研究科
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- 木下 保則
- 埼玉大学大学院理工学研究科
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- Shamim Ahmed
- The Graduate School of Science and Engineering, Saitama University
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- 西垣 功一
- 埼玉大学大学院理工学研究科
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- 内田 秀和
- 埼玉大学大学院理工学研究科
書誌事項
- タイトル別名
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- Improvement of Detection Sensitivity for Small Amount Fluorescence Dyes in Microwell by Confocal Optics System
- キョウ ショウテン コウガクケイ オ モチイタ マイクロウェル ナイ ビリョウ ケイコウタイ ノ ケンシュツ カンド コウジョウ
この論文をさがす
抄録
In this study, we worked on the improvement of detection sensitivity for fluorescence dyes filled in the well of Microwell with Manageable Volume (MMV). For this purpose, we applied fluorescence lifetime imaging microscopy (FLIM) and time-resolved fluorescence (TRF) to method of fluorescence detection. In addition, we built up confocal optics system with a pinhole in front of the photomultiplier tube on the fluorescence microscopy in order to decrease noise from MMV. In the result, the confocal optics system increased the S/N ratio of photon count from dilute Alexa fluor 430. However, the pinhole limited available photon and mechanical vignetting of light path by lateral face of the well also restricted available photon. They caused increasing standard deviation of the result. These issues affected sensitivity of fluorescence detection and it is necessary to balance between noise reduction and amount of available photon for improvement of detection sensitivity by confocal optics system. We suggested suitable pinhole size and other parameters in order to keep the balance, and we succeeded to detect 1 pM Alexa fluor 430 filled into the well of MMV.
収録刊行物
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- 電気学会論文誌E(センサ・マイクロマシン部門誌)
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電気学会論文誌E(センサ・マイクロマシン部門誌) 133 (6), 250-257, 2013
一般社団法人 電気学会
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詳細情報 詳細情報について
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- CRID
- 1390001204462373888
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- NII論文ID
- 10031175337
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- NII書誌ID
- AN1052634X
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- ISSN
- 13475525
- 13418939
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- NDL書誌ID
- 024699543
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
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