Lensless CMOS Imaging Device for Fluorescent and Non-Fluorescent Imaging Dedicated to Digital ELISA

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  • Sasagawa Kiyotaka
    Graduate School of Materials Science, Nara Institute of Science and Technology Japan Science and Technology Agency-CREST
  • Takehara Hironari
    Graduate School of Materials Science, Nara Institute of Science and Technology Japan Science and Technology Agency-CREST
  • Nagasaki Mizuki
    Graduate School of Materials Science, Nara Institute of Science and Technology
  • Takehara Hiroaki
    Graduate School of Materials Science, Nara Institute of Science and Technology Japan Science and Technology Agency-CREST
  • Noda Toshihiko
    Graduate School of Materials Science, Nara Institute of Science and Technology Japan Science and Technology Agency-CREST
  • Tokuda Takashi
    Graduate School of Materials Science, Nara Institute of Science and Technology Japan Science and Technology Agency-CREST
  • Noji Hiroyuki
    Japan Science and Technology Agency-CREST Department of Applied Chemistry, School of Engineering, The University of Tokyo
  • Ohta Jun
    Graduate School of Materials Science, Nara Institute of Science and Technology Japan Science and Technology Agency-CREST

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Other Title
  • デジタルELISAに向けた蛍光および非蛍光計測用レンズレスCMOSイメージングシステム
  • デジタル ELISA ニ ムケタ ケイコウ オヨビ ヒケイコウ ケイソクヨウ レンズレス CMOS イメージング システム

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Abstract

We report a lensless complementary metal-oxide-semiconductor (CMOS) imaging device that can obtain fluorescence and non-fluorescence images for digital enzyme linked immunosorbent assay (ELISA). In lensless imaging, it is important to manage the degradation of the spatial resolution with beam propagation. For fluorescence imaging, we applied an image processing technique based on deconvolution method to recover the spatial resolution. For non-fluorescence imaging, a collimated illumination is used and the detection error is reduced by using an angle scanning technique.

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