Kinetic Approach to the Nonlinear Curve of Bovine Serum Albumin Desorption from Stainless Steel Surfaces
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- TAKAHASHI KAZUHIRO
- Industrial Technological Center of Okayama Prefecture
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- FUKUZAKI SATOSHI
- Industrial Technological Center of Okayama Prefecture
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説明
The kinetics of the desorption of bovine serum albumin (BSA) from stainless steel particles at various surface coverage values (θ) or at various cleaning temperatures were studied in a plug-flow column fed by a 0.1 M NaOH solution.The desorption curves obtained by plotting the logarithm of the amount of residual BSA against cleaning time was analyzed by using an integrated model for two first-order processes occurring simultaneously.At each θ or temperature, the curve of BSA desorption could be successfully reduced to the sum of two independent and simultaneous first-order processes occurring at different rates.The desorption rate constant (kf) for the faster-desorbing BSA (BSAf) was influenced by θ, especially above 0.6.On the other hand, the rate constant (ks) for the slower-desorbing BSA (BSAs) was nearly constant when θ was above 0.6.The ks was approximately 10 to 20 times lower than kf.The Kf showed an Arrhenius-type temperature dependence.The apparent activation energy (Ea) of kf was estimated to be 33kJ/mol.The kf increased by approximately 1.4 times for every 10°C rise in temperature.The proportion of BSAs to the total amount of adsorbed BSA decreased with increasing cleaning temperatures.
収録刊行物
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- Ｂｉｏｃｏｎｔｒｏｌ Ｓｃｉｅｎｃｅ
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Ｂｉｏｃｏｎｔｒｏｌ Ｓｃｉｅｎｃｅ 8 (3), 111-117, 2003
日本防菌防黴学会
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詳細情報 詳細情報について
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- CRID
- 1390001204463824256
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- NII論文ID
- 10011719507
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- NII書誌ID
- AA11169621
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- COI
- 1:CAS:528:DC%2BD3sXos1Wrt7g%3D
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- ISSN
- 18840205
- 13424815
- http://id.crossref.org/issn/13424815
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
