Action of Reactive Oxygen Species in the Antifungal Mechanism of Gemini-pyridinium Salts Against Yeast

DOI PubMed 2 Citations 37 References Open Access
  • SHIRAI AKIHIRO
    Department of Biological Science and Technology, Biosystems Engineering, Institute of Technology and Science, The University of Tokushima
  • UETA SHOUKO
    Department of Biological Science and Technology, Biosystems Engineering, Institute of Technology and Science, The University of Tokushima
  • MASEDA HIDEAKI
    Department of Biological Science and Technology, Biosystems Engineering, Institute of Technology and Science, The University of Tokushima
  • KOURAI HIROKI
    Department of Biological Science and Technology, Biosystems Engineering, Institute of Technology and Science, The University of Tokushima
  • OMASA TAKESHI
    Department of Biological Science and Technology, Biosystems Engineering, Institute of Technology and Science, The University of Tokushima

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  • Action of reactive oxygen species in the antifungal mechanism of gemini-pyridinium salts against yeasts

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We previously found that the gemini quaternary salt (gemini-QUAT) containing two pyridinium residues per molecule, 3,3'- (2,7-dioxaoctane) bis (1-decylpyridinium bromide) (3DOBP-4,10) , exerted fungicidal activity against Saccharomyces cerevisiae and caused respiration inhibition and the cytoplasmic leakage of ATP, magnesium, and potassium ions. Here, we investigated how the gemini-QUAT, 3DOBP-4,10, exerts more powerful antimicrobial activity than the mono-QUAT N-cetylpyridinium chloride (CPC) and examined the association between reactive oxygen species (ROS) and the antimicrobial mechanism. Antifungal assays showed that the activity of 3DOBP-4,10 against two yeasts, S. cerevisiae and Candida albicans, was significantly elevated under aerobic conditions, and largely reduced under anaerobic conditions (nitrogen atmosphere) . Adding radical scavengers such as superoxide dismutase, catalase and potassium iodide (KI) also decreased the fungicidal activity of 3DOBP-4,10 but negligibly affected that of CPC. We measured survival under static conditions and found that the rapid fungicidal profile of 3DOBP-4,10 was lost, whereas that of CPC was slightly affected in the presence of KI. Our results suggest that 3DOBP-4,10 exerts powerful antimicrobial activity by penetrating the cell wall and membrane, which then allows oxygen to enter the cells, where it participates in the generation of intracellular ROS. The activity could thus be attributable to a synergic antimicrobial combination of the disruption of organelle membranes by the QUAT and oxidative stress imposed by ROS.

Journal

  • Biocontrol Science

    Biocontrol Science 17 (2), 77-82, 2012

    The Society for Antibacterial and Antifungal Agents, Japan

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