Comparison of Chromogenic Selective Media for the Detection of <i>Cronobacter</i> spp. (<i>Enterobacter sakazakii</i>)
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- TERAMURA HAJIME
- Yokohama Research Center, JNC Corporation Department of Food Bioscience and Biotechnology, College of Bioresource Science, Nihon University
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- FUKUDA NORIKO
- Department of Food Bioscience and Biotechnology, College of Bioresource Science, Nihon University
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- OKADA YUMIKO
- Division of Biomedical Food Research, National Institute of Health Science
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- OGIHARA HIROKAZU
- Department of Food Bioscience and Biotechnology, College of Bioresource Science, Nihon University
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説明
<p> The four types of chromogenic selective media that are commercially available in Japan were compared for establishing a Japanese standard method for detecting Cronobacter spp. based on ISO/TS 22964:2006. When assessed using 9 standard Cronobacter spp. strains and 29 non-Cronobacter strains, Enterobacter sakazakii isolation agar, ChromocultTM Enterobacter sakazakii agar, CHROMagarTM E. sakazakii, and XM-sakazakii agar demonstrated excellent inclusivity and exclusivity. Using the ISO/TS 22964:2006 method, the recovered numbers of 38 Cronobacter spp. strains, including 29 C. sakazakii isolates obtained from each medium, were equivalent, indicating that there was no significant difference (p > 0.05) among the four types of chromogenic selective media. Thus, we demonstrated that these four chromogenic selective media are suitable alternatives when using the standard method for detecting Cronobacter spp. in Japan, based on the ISO/TS 22964:2006.</p>
収録刊行物
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- Ｂｉｏｃｏｎｔｒｏｌ Ｓｃｉｅｎｃｅ
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Ｂｉｏｃｏｎｔｒｏｌ Ｓｃｉｅｎｃｅ 23 (1), 27-33, 2018
日本防菌防黴学会
