アガロースゲル電気泳動法によるウズラ赤血球酵素 (PGI, 6PGD, PGM, Es-D) の同時検出法

DOI

書誌事項

タイトル別名
  • A Method of Simultaneous Detection of Several Erythrocyte Isozymes (PGI, 6PGD, PGM, Es-D) in Quail by Agarose Gel Electrophoresis

抄録

Phosphoglucose isomerase (PGI), 6-Phosphogluconate dehydrogenase (6PGD), Phosphoglucose mutase (PGM) and Esterase-D (Es-D) isozymes were possible to detect simultaneously in the same agarose gel by electrophoresis in a continuous phosphate buffer system at pH 7.8. Electrophoresis was carried out at 16v/cm for 120 minutes followed by at 20v/cm for 10 minutes. The cooling (10°C) of the gel at electrophoresis was the most important in this method. The mixture of substrates for each isozyme in the same buffer (0.1 M Tris-HCI pH 8.0) was available to detect four isozymes simultaneously.<BR>The advantages of this method are as follow 1) 3 or 4 isozymes which have a similar optimal pH in a reaction are detected in one agerose gel simultaneneously. 2) More than 40 samples can be analysed in 2 or 3 rows on one agarose gel. 3) Only small amount of reaction mixture is needed (10 ml per 21 × 23 cm of gel) . 4) The time required for detection is short.

収録刊行物

キーワード

詳細情報 詳細情報について

  • CRID
    1390001204467142912
  • NII論文ID
    130003857546
  • DOI
    10.5924/abgri1993.21.33
  • ISSN
    18843875
    09194371
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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