Quantitative Evaluation of Protective Effect of Pb1 Gene, Conferring Field Resistance to Rice Panicle Blast, Using Near-Isogenic Lines

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  • Fujii Kiyoshi
    Aichi Prefectural Agricultural Research Center (AARC)
  • Hayano-Saito Yuriko
    National Agricultural Research Center for Hokkaido Region
  • Sugiura Naoki
    Aichi Prefectural Agricultural Research Center (AARC) Present address: Aichi Prefectural College of Agriculture
  • Hayashi Nagao
    Mountainous Regional Agriculture Research Institute, AARC Present address: National Institute of Agrobiological Resources
  • Izawa Toshihiko
    Aichi Prefectural Agricultural Research Center (AARC)
  • Iwasaki Mabito
    National Agricultural Research Center for Hokkaido Region

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Other Title
  • イネ準同質遺伝子系統を用いた穂いもち圃場抵抗性遺伝子Ｐｂ１による穂いもち発病抑制効果の定量的評価
  • イネ ジュンドウシツ イデンシ ケイトウ オ モチイタ ホイモチ ホジョウ テイコウセイ イデンシ Pb1 ニ ヨル ホイモチ ハツビョウ ヨクセイ コウカ ノ テイリョウテキ ヒョウカ

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Abstract

To evaluate quantitatively the protective effects of the Pb1 gene which is derived from an Indica rice variety “Modan” and confers field resistance to rice panicle blast, tests for blast resistance were conducted in paddy fields over a period of two years at two testing sites using three pairs of near-isogenic lines (NILs) for the Pb1 locus selected from about 2,000 recombinant inbred lines (RILs) which were developed from the cross between the Japanese rice cultivars “Koganebare” (susceptible to rice blast without Pb1 gene) and “Tsukinohikari” (resistant to the disease with Pb1). A NIL pair of cultivar “Koshihikari” for Pb1 and Stvb-i, a gene conferring resistance to rice stripe virus, was also used for the evaluation, in which the protective effect was given as follows: <br><br>Protective effect<br>=(rate of infected part without Pb1−rate of infected part with Pb1)/(rate of infected part without Pb1)×100<br><br> The protective effect of the Pb1 gene against rice blast increased with the progression of the growth stages of the rice plants; leaf (vegetative growth) stage < flag leaf stage < panicle (reproductive growth) stage. The average protective value of the Pb1 gene for the percentage of diseased grains was very high (93) for each year, which showed that Pb1 could reduce the percentage of diseased grains to less than 1/10, compared with sib-lines without Pb1 gene. The head brown rice yield ratios of NILs with Pb1 to the sib-lines without Pb1 were 2.40:1-16.2:1 under conducive conditions for blast disease, revealing a very high protective effect on yield loss associated with panicle blast infection, while 0.78:1-1.29:1 ((1.07± 0.18):1) under conditions less conducive to the disease. The values of the head/gross brown rice rate and thousand kernel weight in brown rice of the NILs with Pb1 under disease-conducive conditions, were significantly higher, while the protein content was significantly lower than the values of the sib-lines without Pb1, respectively. Thus, the Pb1 gene also showed a secondary protective effect on rice quality under the blast fungus-conducive environment.<br>

Journal

  • Breeding Research

    Breeding Research 7 (2), 75-85, 2005

    Japanese Society of Breeding

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