Comparison in Quantification of Human Immunodeficiency Virus Type 1 RNA Measured by Standard Method (Amplicor HIV Monitor Test Ver.1.0) and Add-in Primer Mix Method
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- NISHIDA Yasuharu
- Department of Clinical Pathology, Tokyo Medical University
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- MORIYA Kenji
- Department of Clinical Pathology, Tokyo Medical University
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- KITANO Kiyoshi
- Second Department of Internal Medicine, Shinshu University, School of Medicine
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- MAMIYA Shigeo
- Third Department of Internal Medicine, Akita University, School of Medicine
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- NAGAIZUMI Keiko
- Department of Clinical Pathology, Tokyo Medical University
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- INABA Hiroshi
- Department of Clinical Pathology, Tokyo Medical University
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- AMANO Kagehiro
- Department of Clinical Pathology, Tokyo Medical University
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- FUKUE Hidetaka
- Department of Clinical Pathology, Tokyo Medical University
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- YAMAMOTO Yasuyuki
- Department of Clinical Pathology, Tokyo Medical University
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- ARAI Morio
- Department of Clinical Pathology, Tokyo Medical University
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- FUKUTAKE Katsuyuki
- Department of Clinical Pathology, Tokyo Medical University
Bibliographic Information
- Other Title
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- Human Immunodeficiency Virus Type 1 RNA定量における現行法(アンプリコアHVI‐1モニターVer1.0)とアド・イン・プライマー法の解離に関する検討
- Human Immunodeficiency Virus Type 1 RNA テイリョウ ニ オケル ゲンコウホウ アンプリコア HIV 1 モニター Ver 1 0 ト アド イン プライマーホウ ノ カイリ ニ カンスル ケントウ
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Abstract
Objective: The level of human immunodeficiency virus type 1 (HIV-1) RNA in plasma is one of the important parameters in clinical practices for HIV-1 infected patients. How ver, it has been suggested that the standard method (Amplicor HIV Monitor Test Ver1.0) is less sensitive to non-subtype B than subtype B HIV-1. We therefore examined the usefulness of the add-in primer mix method using samples obtained from Japanese HIV-1 infected patients.<BR>Method: We compared the sensitivity of the standard method and that of a modified method with an add-in primer, using plasma samples obtained from 12 seropositive hemophiliacs and 82 seropositive non-hemophiliacs.<BR>Results: Cross-reactivity was less than 12.1% on average in 17 (32.1%) of the 53 non-hemophiliac samples when comparing standard and modified methods, while the HIV-1 RNA levels determined with these two methods were comparable in the 12 hemophiliacs.<BR>Conclusion: These results indicate that the HIV-1 RNA assay system, which has high sensitivity not only to subtype B but also to non-subtype B HIV-1, is inevitable in Japan.
Journal
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- The Journal of AIDS Research
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The Journal of AIDS Research 2 (2), 85-88, 2000
The Japanese Society for AIDS Research
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Details 詳細情報について
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- CRID
- 1390001204470489088
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- NII Article ID
- 10020441469
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- NII Book ID
- AA11379941
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- ISSN
- 18842763
- 13449478
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- NDL BIB ID
- 5407576
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed