Analyzing TOF-SIMS spectra of biopolymer using multivariate curve resolution
-
- Aoyagi Satoka
- Faculty of Life and Environmental Science, Shimane University Division of Production Environment Engineering, United Graduate School of Agricultural Sciences, Tottori University
-
- Okamoto Masayuki
- Division of Production Environment Engineering, United Graduate School of Agricultural Sciences, Tottori University Kao Corporation
-
- Kato Nobuhiko
- Faculty of Science and Technology, Seikei University
-
- Kudo Masahiro
- Faculty of Science and Technology, Seikei University
この論文をさがす
抄録
Fragment ion analysis of protein samples is crucial for structural evaluation of proteins. Interpretation of fragment ions from bioplolymers, however, is often difficult because fragmentation mechanisms in secondary ion mass spectrometry have not been clarified. Therefore employment of chemometrics is necessary to obtain detailed information from time-of-flight secondary ion mass spectrometry (TOF-SIMS) data of complex samples. In this study, multivariate curve resolution (MCR) was applied to extract a pure component spectrum from a TOF-SIMS spectrum which contains mixed materials information. The model biopolymer, chicken egg white lysozyme was immobilized on a gulutaraldehyde-activated aminosilanized indium-tin oxide (ITO) glass plate by covalent bonding. A reference sample such as substrates without the protein were prepared. All samples were measured with TOF-SIMS using Bi3+, and then the TOF-SIMS spectra data were analyzed using MCR with and without data preprocessing. As a result, images and spectra related to each material in the sample were obtained by MCR. The fragment ions in the extracted spectra are consistence with chemical information of each material.
収録刊行物
-
- Journal of Surface Analysis
-
Journal of Surface Analysis 17 (3), 220-223, 2011
一般社団法人 表面分析研究会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001204471315328
-
- NII論文ID
- 130005138943
-
- NII書誌ID
- AA11448771
-
- ISSN
- 13478400
- 13411756
- http://id.crossref.org/issn/13411756
-
- NDL書誌ID
- 11077487
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可