Chemical imaging of biomolecules in skin using TOF-SIMS and multivariate analysis

DOI Web Site Web Site 被引用文献2件 参考文献8件
  • Aoyagi Satoka
    Faculty of Life and Environmental Science, Shimane University
  • Matsuzaki Takashi
    Faculty of Life and Environmental Science, Shimane University
  • Kato Nobuhiko
    Faculty of Life and Environmental Science, Shimane University Faculty of Science and Technology, Seikei University
  • Kudo Masahiro
    Faculty of Science and Technology, Seikei University

この論文をさがす

抄録

Chemical mapping of biomolecules in biological samples such as tissues and cells is crucial for biological, medical and biochemical fields. Although secondary ion images of biomolecules such as phosphocholine and cholesterol distributions on tissue samples are easily obtained with time-of-flight secondary ion mass spectrometry (TOF-SIMS), the observation of minor ingredients is often difficult. In this study TOF-SIMS imaging data of skin samples were analyzed with a multivariate analysis technique, multivariate curve resolution (MCR), in order to investigate appropriate analysis conditions. Mice middorsal skin was sliced with a microtome and placed on indium-tin oxide glass plates. The samples were measured with TOF-SIMS using Bi3+, and then the obtained data were analyzed with MCR under various analysis conditions for investigating appropriate conditions. As a result, it is indicated that MCR classify TOF-SIMS raw data into some categories representing different features, which makes interpretation of TOF-SIMS data easier.

収録刊行物

被引用文献 (2)*注記

もっと見る

参考文献 (8)*注記

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ