コウノトリ <i>Ciconia boyciana</i> の羽根1本からの抽出DNAを用いたPCR法による性鑑別

  • MURATA Koichi
    Kobe Municipal Oji Zoo
  • ITOH Yuichiro
    Laboratory of Molecular Biology, Department of Applied Biological Chemistry
  • OGAWA Akira
    Laboratory of Molecular Biology, Department of Applied Biological Chemistry
  • MIZUNO Shigeki
    Laboratory of Molecular Biology, Department of Applied Biological Chemistry

書誌事項

タイトル別名
  • Sexing the Oriental White Stork Ciconia boyciana by PCR Using a Single Plucked Feather as a Source of DNA

抄録

A small amount of DNA extracted from a single plucked contour feather from four captive young Oriental White Storks Ciconia boyciana, a sexually monomorphic bird, was used for gender determination by the method of polymerase chain reaction (PCR) based on its sex chromosome-specific DNA sequences, the 0.6-kb Xba I -HindIII fragment(XH0.6) on the W chromosome and the region homologous to the XH0.6sequence (XH0.6-RSM) on the Z chromosome. After the agarose gel electrophoresis of the PCR products, two bands which correspond to the W and Z chromosome-specific fragments were observed from the female DNA. By contrast only one Z chromosome-specific fragment was observed from the male DNA. These results were consistent with those obtained by Southern blot hybridization using the DNA from red blood cells and the discriminant analysis of body measurements. This PCR method using a single feather is easy, fast, accurate, and also relatively stress-less to the birds which are not used to being handled for sampling. It will become one of useful tools in the captive propagation program and reintroduction plan for the C. boyciana, a special natural monument of Japan.

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詳細情報 詳細情報について

  • CRID
    1390001204480787328
  • NII論文ID
    130003564625
    30037479468
  • DOI
    10.3838/jjo.46.157
  • ISSN
    18819710
    0913400X
    http://id.crossref.org/issn/0913400X
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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