Sexing the Oriental White Stork Ciconia boyciana by PCR Using a Single Plucked Feather as a Source of DNA

MURATA Koichi, ITOH Yuichiro, OGAWA Akira, MIZUNO Shigeki

doi:10.3838/jjo.46.157

Bibliographic Information

Other Title

コウノトリ <i>Ciconia boyciana</i> の羽根1本からの抽出DNAを用いたPCR法による性鑑別

Abstract

A small amount of DNA extracted from a single plucked contour feather from four captive young Oriental White Storks Ciconia boyciana, a sexually monomorphic bird, was used for gender determination by the method of polymerase chain reaction (PCR) based on its sex chromosome-specific DNA sequences, the 0.6-kb Xba I -HindIII fragment(XH0.6) on the W chromosome and the region homologous to the XH0.6sequence (XH0.6-RSM) on the Z chromosome. After the agarose gel electrophoresis of the PCR products, two bands which correspond to the W and Z chromosome-specific fragments were observed from the female DNA. By contrast only one Z chromosome-specific fragment was observed from the male DNA. These results were consistent with those obtained by Southern blot hybridization using the DNA from red blood cells and the discriminant analysis of body measurements. This PCR method using a single feather is easy, fast, accurate, and also relatively stress-less to the birds which are not used to being handled for sampling. It will become one of useful tools in the captive propagation program and reintroduction plan for the C. boyciana, a special natural monument of Japan.

Journal

Japanese Journal of Ornithology

Japanese Journal of Ornithology 46 (3), 157-162, 1998

The Ornithological Society of Japan

Keywords

Details 詳細情報について

CRID: 1390001204480787328

NII Article ID: 130003564625; 30037479468

DOI: 10.3838/jjo.46.157

ISSN: 18819710; 0913400X; http://id.crossref.org/issn/0913400X

Text Lang: en

Data Source

JaLC
Crossref
CiNii Articles

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