Analysis of Cannabinoids by HPLC Using a Post-Column Reaction with Fast Blue B Salt.

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  • Chinaka Satoshi
    Forensic Science Laboratory, Ishikawa Prefectural Police Headquarters
  • Tanaka Seishi
    Forensic Science Laboratory, Ishikawa Prefectural Police Headquarters
  • Takayama Nariaki
    Forensic Science Laboratory, Ishikawa Prefectural Police Headquarters
  • Ueda Kazumasa
    Department of Chemistry and Chemical Engineering, Faculty of Engineering, Kanazawa University

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Abstract

  Three cannabinoids, Δ9-tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN), were detected with an HPLC method using a post-column reaction with Fast Blue B salt (FBB). The detection limit of THC was 0.1 ng, which is ca. 1000 times more sensitive than TLC using FBB coloration. The calibration curve of THC was linear in the range 0.01-100 μg/ml, and the intermediate precisions of within-run and between-run assays were 0.93% and 1.12%, respectively. The proposed method is highly selective and quantitative.<br>

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