Mobility of Drugs in Lipid Membranes by NMR

DOI Web Site Web Site 3 References

Bibliographic Information

Other Title
  • NMRによる脂質膜中の薬物の「運動」の解析
  • NMR ニ ヨル シシツ マク チュウ ノ ヤクブツ ノ ウンドウ ノ カイセキ

Search this article

Description

Mobility of drugs and biomembrane constituents is a key to elucidate the membrane transport mechanism in the cell. Lipid bilayer membrane is a dynamic structure where molecules are always fluctuating under physiological conditions. The mechanism of drug transport is related to the molecular dynamics in such soft, fluid membrane interface. To gain insight into molecular movements in membranes, we develop a noninvasive method to monitor dynamic properties of drugs and lipid components in membranes by applying multinuclear high-resolution solution NMR in combination with the pulsed-field-gradient (PFG) technique. We have quantified the diffusivity, the kinetics of membrane binding, and the bound fraction of the drug in situ by using large unilamellar vesicles of egg phosphatidylcholine as model cell membranes. The combination of 1D and PFG NMR serves to quantify the kinetics of membrane binding where the bound and the free components are unable to distinguish because of the rapid exchange on the NMR timescale. A small-sized 5-fluorouracil and fluorinated bisphenol A are used as a model drug.

Journal

  • Oleoscience

    Oleoscience 11 (6), 213-220, 2011

    Japan Oil Chemists' Society

References(3)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top