Tridimensional Response of human Dental Follicular Stem Cells onto a Synthetic Hydroxyapatite Scaffold
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- Mastrangelo Filiberto
- Department of Stomatology and Oral Science, Division of Oral Surgery, University “G. d'Annunzio,”
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- Nargi Elena
- Department of Biomedical Scienze, Division of Pharmacology and Toxicology, University “G. d'Annunzio,”
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- Carone Luigi
- Department of Stomatology and Oral Science, Division of Oral Surgery, University “G. d'Annunzio,”
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- Dolci Marco
- Department of Stomatology and Oral Science, Division of Oral Surgery, University “G. d'Annunzio,”
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- Caciagli Francesco
- Department of Biomedical Scienze, Division of Pharmacology and Toxicology, University “G. d'Annunzio,”
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- Ciccarelli Renata
- Department of Biomedical Scienze, Division of Pharmacology and Toxicology, University “G. d'Annunzio,”
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- Lutiis Maria Anna De
- Biology Division, University “G. d'Annunzio,”
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- Karapanou Virginia
- Department of Endodontics, School of Dental Medicine, Tufts University
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- Shaik Basha Y.
- Department of Oral Biology, Dental Medicine, Boston University
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- Conti Pio
- Immunology Division, University “G. d'Annunzio,”
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- Teté Stefano
- Department of Stomatology and Oral Science, Division of Oral Surgery, University “G. d'Annunzio,”
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In the last decade, extracorporeal bone tissue engineering has found more clinical applications due to the progress and new achievements in the isolation and characterization of stem cells from different sources, as well as, in controlling proliferation and differentiation in vitro. The aim of this study is to evaluate the in vitro behaviour, morphological structure and extracellular matrix synthesis of human dental follicle stem cells (hDFSCs) isolated from human dental bud, when seeded onto a synthetic hydroxyapatite (HA) scaffold (ENGIpore©). Populations of CD29+, CD90+, CD146+ and CD166+ were sorted by FAC sorter (FACS) analysis and were cultured in osteogenic medium and then, onto the scaffold. These cells were analyzed by optical and electronic microscopy, at week 1 and 6, before and after the differentiation. Light microscopy showed an intense attachment and colonization of the HA scaffold by polygonal-shaped cells. Scanning electron microscopy after six weeks revealed a tri-dimensional organization of the cells and the presence of dense material around the cell clusters. hDFSCs showed participation in protein biosynthesis and demonstrated high proliferation on the synthetic HA scaffold.
収録刊行物
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- JOURNAL OF HEALTH SCIENCE
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JOURNAL OF HEALTH SCIENCE 54 (2), 154-161, 2008
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204497551872
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- NII論文ID
- 110006649665
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- NII書誌ID
- AA11316464
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- ISSN
- 13475207
- 13449702
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- NDL書誌ID
- 9441264
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可