Coffee Induces 11.BETA.-Hydroxysteroid Dehydrogenase Type 2 Activity in Human Placental Choriocarcinoma Cells, JEG-3
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- Arao Tomomi
- The Graduate School of Pharmaceutical Sciences, Keio University
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- Tamura Hiroomi
- The Graduate School of Pharmaceutical Sciences, Keio University
Bibliographic Information
- Other Title
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- Coffee induces 11β-hydroxysteroid dehydrogenase type 2 activity in human placental choriocarcinoma cells JEG-3
- Coffee induces 11 v hydroxysteroid dehydrogenase type 2 activity in human placental choriocarcinoma cells JEG 3
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Abstract
Coffee is the beverage consumed during pregnancy that is most commonly associated with adversely affecting the developing fetoplacental unit. Since placental 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD-2) is an important determinant for maintenance of fetal glucocorticoid levels during pregnancy, we investigated the effect of coffee on 11β-HSD-2 expression in the human placental choriocarcinoma cell line, JEG-3. Addition of coffee to the culture medium of JEG-3 cells increased 11β-HSD-2 gene expression after 24hr in a dose-dependent manner. Cellular 11β-HSD-2 activity, which was calculated by the amount of cortisol that was converted to cortisone in the culture medium, also increased in the coffee-treated JEG-3 cells. None of the major constituents of coffee, such as caffeine, caffeic acid or chlorogenic acid, showed any ability to induce 11β-HSD-2 gene expression. The 11β-HSD-2-inducing activity in coffee was extractable with ethyl acetate. Increasing the degree of roasting improved the ability of the coffee extract to induce 11β-HSD-2 gene expression indicating that the active constituent(s) is produced during the roasting process.
Journal
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- Journal of Health Science
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Journal of Health Science 57 (5), 436-441, 2011
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390001204498370304
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- NII Article ID
- 130001151386
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- NII Book ID
- AA11316464
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- ISSN
- 13475207
- 13449702
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- NDL BIB ID
- 11250209
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed