Mutagenicities and Endocrine-disrupting Activities of 1-Hydroxy-2-nitropyrene and 1-Hydroxy-5-nitropyrene
-
- Kameda Takayuki
- Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University
-
- Akiyama Ayuko
- Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University
-
- Yoshita Morio
- Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University
-
- Tachikawa Chihiro
- Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University
-
- Toriba Akira
- Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University
-
- Tang Ning
- Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University
-
- Hayakawa Kazuichi
- Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University
この論文をさがす
抄録
The mutagenicities and endocrine-disrupting activities of two isomers of mononitrated 1-hydroxypyrene [1-hydroxy-x-nitropyrenes (1-OH-x-NPs); x=2 and 5], which are not only photoreaction products of 1-nitropyrene (1-NP) but also constituent of ambient airborne particles, were evaluated for the first time using the Ames plate incorporation assay and the yeast two-hybrid assay, respectively. The mutagenicity of 1-OH-5-NP was weakly positive in the absence of rat liver S9, but was enhanced up to 3-fold with the metabolic activation by S9. On the contrary, 1-OH-2-NP did not exhibit significant mutagenicity in the presence or absence of S9. 1-OH-5-NP showed weak estrogenic activity, but 1-OH-2-NP did not show any estrogenic activity. The concentration of 1-OH-5-NP that gave 10% of activity of 1.0×10-6 M 17β-estradiol (E2) was 5.4×10-7 M. 1-OH-5-NP exhibited stronger antiestrogenic and antiandrogenic activities than 1-OH-2-NP. 1-OH-5-NP at a concentration of 1.0×10-6 M inhibited 71 and 90% of β-galactosidase activity induced by 1.0×10-9 M of E2 and 1.0×10-8 M of 5α-dihydrotestosterone (DHT), respectively. On the other hand, 1.0×10-6 M of 1-OH-2-NP inhibited 16 and 43% of β-galactosidase activity induced by 1.0×10-9 M of E2 and 1.0×10-8 M of DHT, respectively. These findings point out the need for determining the environmental sources and distribution of 1-OH-2-NP and 1-OH-5-NP as well as the other hydroxynitropyrene isomers.
収録刊行物
-
- JOURNAL OF HEALTH SCIENCE
-
JOURNAL OF HEALTH SCIENCE 57 (4), 372-377, 2011
公益社団法人 日本薬学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001204498552576
-
- NII論文ID
- 130001001083
-
- NII書誌ID
- AA11316464
-
- COI
- 1:CAS:528:DC%2BC3MXhtV2gu7zP
-
- ISSN
- 13475207
- 13449702
-
- HANDLE
- 2297/29005
-
- NDL書誌ID
- 11173947
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可