Production and Application of Monoclonal Antibodies to Human Selenoprotein P.
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- Saito Yoshiro
- Department of Hygienic Chemistry, Graduate School of Pharmaceutical Science, Hokkaido University
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- Watanabe Yasuko
- Department of Hygienic Chemistry, Graduate School of Pharmaceutical Science, Hokkaido University
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- Saito Eiji
- Second Department of Internal Medicine, Nihon University School of Medicine
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- Honjoh Tsutomu
- Morinaga Institute of Biological Sciences
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- Takahashi Kazuhiko
- Department of Hygienic Chemistry, Graduate School of Pharmaceutical Science, Hokkaido University
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Abstract
Selenoprotein P is a selenium-rich extracellular protein and is the major selenoprotein in plasma. Although the biological function of selenoprotein P has not been established, we have recently shown that selenoprotein P has phospholipid hydroperoxide glutathione peroxidase-like activity. To study the structure and function of selenoprotein P, we produced monoclonal antibodies against human selenoprotein P. Immunization of rats with purified selenoprotein P was followed by hybridization, cloning, and the establishment of eleven hybridomas producing specific antibodies against human selenoprotein P. With the addition of six kinds of insoluble rat anti-human selenoprotein P monoclonal antibodies to human plasma, the selenium concentration of the supernatant was decreased to 47% of the control. This suggests that selenoprotein P constituted 53% of total plasma selenium. Western blot analysis of the immunoprecipitate from human plasma showed that the antibodies specifically bound to a 69 kDa protein, representing selenoprotein P. Next, we developed an enzyme-linked immunosorbent assay for selenoprotein P using two monoclonal antibodies. The plasma concentration of selenoprotein P in 77 normal individuals was 5.3 ± 1.1 μg/ml. Because preferential depletion of selenoprotein P by low density lipoprotein (LDL)-apheresis has been reported, we next measured selenoprotein P concentration of plasma samples from patients before and after LDL-apheresis. We confirmed that the plasma concentration of selenoprotein P was decreased from 5.7 to 2.3 μg/ml by LDL-apheresis. This result shows that this assay provides a reliable means of measuring the content of selenoprotein P in plasma.
Journal
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- Journal of Health Science
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Journal of Health Science 47 (4), 346-352, 2001
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390001204499212288
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- NII Article ID
- 110003641415
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- NII Book ID
- AA11316464
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- COI
- 1:CAS:528:DC%2BD3MXlslynurY%3D
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- ISSN
- 13475207
- 13449702
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- NDL BIB ID
- 5852381
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed