New method for evaluation of regulatory agents on the skin by using fibroblasts culturing within collagen gel. (II). Correlation between contraction of collagen gel and morphological change of cells.

DOI

Bibliographic Information

Other Title
  • 線維芽細胞を用いた皮膚賦活剤評価法の開発　ＩＩ　　コラーゲンゲル収縮と細胞形態との相関
  • Correlation between contraction of collagen gel and morphological change of cells
  • コラーゲンゲル収縮と細胞形態との相関

Abstract

Fibroblast like cells (swiss 3T3 cells) treated by trypsin under various conditions were cultured within collagen gel. Contraction of collagen gel was more retarded with cells treated as increasing concentration of trypsin or as increasing time of trypsin treatment. Number of spherical cells within collagen gel observed by phase-contrast micrography 6hr after culture increased with increasing concentration of trypsin or time of trypsin treatment, while bipolar cells decreased. These results suggest that cell surface contains a membrane protein which is trypsin-sensitive and which interacts with collagen, ultimately contributing to contraction of collagen gel. Correlation between contraction of collagen gel and morphological change of cells might be realized by the membrane protein assumed to exist. It seems that the delay of contraction by trypsin treatment of cells is related to a prolonged period during which the shape of cells changes from sphere to bipolar.

Journal

Details 詳細情報について

  • CRID
    1390001204501634432
  • NII Article ID
    130004188604
  • DOI
    10.5107/sccj.22.263
  • ISSN
    18844146
    03875253
  • Text Lang
    ja
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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