Application of dialysis culture to L-lysine fermentation.
-
- NOMURA Yoshiyuki
- Department of Applied Microbial Technology, The Kumamoto Institute of Technology
-
- IWAHARA Masayoshi
- Department of Applied Microbial Technology, The Kumamoto Institute of Technology
-
- HONGO Motoyoshi
- Department of Applied Microbial Technology, The Kumamoto Institute of Technology
Bibliographic Information
- Other Title
-
- 透析培養法のL‐リジン発酵への応用
- トウセキ バイヨウホウ ノ L リジン ハッコウ ヘ ノ オウヨウ
Search this article
Abstract
In Brevibacterium flavum QL-5, aspartate kinase was sensitive to feedback inhibition in the simultaneous presence of L-lysine and L-threonine. The simultaneous addition of these two amino acids (1mM each) produced about 60% inhibition. The inhibition was reduced to about 40% by dialysis. Similarly in L-lysine production by resting cells, the simultaneous addition of these two amino acids (1mM each) produced 35% inhibition, and dialysis reduced the inhibition to 12%.<br> Dialysis culture was used in L-lysine production by Brevibacterium flavum QL-5 with 10%_??_20% glucose concentration. In dialysis cultures, the lag phase was shortened and cell mass increased compared with non-dialysis cultures. Moreover in dialysis cultures, L-lysine was produced early, and the maximum productivity of L-lysine (1.50g/l/hr) was obtained in 6 through 10 hr cultivation. The maximum productivity in dialysis cultures was 2_??_6 times higher than in non-dialysis cultures at the same culture period. The amounts of L-lysine produced in dialysis culture at 10%, 15% and 20% glucose concentrations increased 105%, 120% and 126%, respectively, as compared with non-dialysis culture at the same glucose concentration. In all dialysis cultures, the yield of L-lysine per glucose consumed was about 30%.
Journal
-
- Nippon Nōgeikagaku Kaishi
-
Nippon Nōgeikagaku Kaishi 61 (8), 957-962, 1987
Japan Society for Bioscience, Biotechnology, and Agrochemistry
- Tweet
Details 詳細情報について
-
- CRID
- 1390001204505103104
-
- NII Article ID
- 130001229449
-
- NII Book ID
- AN00196191
-
- ISSN
- 18836844
- 00021407
-
- NDL BIB ID
- 3144273
-
- Text Lang
- ja
-
- Data Source
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- Abstract License Flag
- Disallowed