Change in DNA Bases, Deoxynucleosides and Deoxynucleotides by γ-Irradiation

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  • KOBAYASHI Tsuya
    Department of Agricultural Chemistry, College of Agriculture and Veterinary Medicine, Nihon University
  • SHIRAI Kazuo
    Department of Agricultural Chemistry, College of Agriculture and Veterinary Medicine, Nihon University

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Other Title
  • γ線照射によるＤＮＡ塩基，デオキシヌクレオシドおよびデオキシヌクレオチドの変化
  • ガンマセン ショウシャ ニヨル DNA エンキ デオキシヌクレオシド オヨビ

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Abstract

Aqueous solutions of DNA bases, deoxynucleosides, deoxynucleotides, and mixtures of DNA base and deoxyribose were γ-irradiated in the presence of air or in nitrogen atmosphere. Each solution was prepared at a concentration of 5×10-4M.<br> UV absorption spectra were measured directly on the test solutions, while the remaining substances and unaltered liberated bases were determined after paper chromatographic separation. The UV absorption spectra were not always depressed with decreases in remaining substances when treated with higher irradiated dose level. Adenine and cytosine appeared to be more stable in free form than in deoxynucleoside or deoxynucleotide form in nitrogen atmosphere.<br> Ammonia and inorganic phosphate were also determined. Ammonia formation was low from thymine, thymidine and thymidine monophosphate, which have no amino group in their side chains. It will be noted that nitrogen in ring scarcely contributes to ammonia formation. Inorganic phosphate formation from nucleoside of pyrimidines was greater than from that of purines.<br> It was observed by CD spectrometry that the conformation change of deoxycytidine by γ-irradiation was greater than that of deoxycytidine 5'-monophosphate.

Journal

  • Nippon Nōgeikagaku Kaishi

    Nippon Nōgeikagaku Kaishi 54 (7), 517-525, 1980

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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